Technical data sheet (Anaplasma phagocytophila)

Ready to use PCR kit for Anaplasma (Ehrlichia) phagocytophila (gel agarose based) for 100 reactions

Anaplasma phagocytophila (AP) is intracellular pathogen, which is cause of interest for causing diseases in human, animals like horses, dogs etc and wild life. It is tick borne micro organism, which is transmitted through the bite of the tick like Ixodes. In human being, it causes human granulocytic ehrlichiosis. It causes blood related disease in animals. It is found as a complex with Babesia and Borrelia in ticks. The role of such complex is under investigation. To eradicate this bacterium, one has to identify this in the samples and there are a number of methods available to detect this bacterium like serological methods. The serological methods have the problem of cross reactions as well as low sensitivity. However one can use other methods like Staining and cell culture methods. The culture method is time consuming therefore the method use should be PCR method, where one can hope to detect the bacterium in the smallest samples with highest sensitivity and specificity.

Target gene: highly conserved region of of genes of AP responsible for encoding protein antigen with ankyrin repeat motifs

Samples: blood, serum, plasma, ticks, tissue, other body fluid samples, cell cultures etc.. This assay needs the isolated good quality DNA, which can be isolated with good commercially available kits as well as home made method.

Key point: This assay has been checked for the cross reactions with related and non related micro organisms like Babesia, Borrelia burgdorferi, Rickettsia, Neospora caninum, Treponema pallidum etc.Ureaplasma

Components: primers, polymerase, nucleotides, buffer, molecular water, loading dye, molecular marker (gene ruler), negative control, positive control and manual. In order to use the internal control: one can use the Genekam species specific DNA identification kit for human for human probes or canine for dog probes or other species.

Detection limit: around 1 CFU per ml

Storage: -20 degree

In vitro use only
Only for research use (for human probes)
To be used through technical persons.