Technical data sheet (Babesia bovis)

Technical details for ready to use PCR kit for Babesia bovis (gel agarose); 100 Reactions

Bovine babesiosis is a tick-borne disease found worldwide in cattle, which is caused by Babesia. After the acute infection (primary infection), recovered animal carries this blood parasite subclinically and it makes these animals undetectable with microscopic diagnosis. These animals serve as carriers, which act later as reservoir for the infection in the herd and continue infecting the tick vectors, which spread the parasite. Therefore it is essential to screen the animals (carriers) with highly sensitive and specific tests. Microscopic diagnosis can not offer such sensitivity as well as serological tests have the problem of cross reactions. The cell culture needs too long time for detection and needs cell culture laboratory. Therefore PCR is merging as gold standard in the field of medicine as the PCR tests are highly sensitive, specific as well as can detect Babesia bovis in one drop of blood also. The PCR can be also used for animals, which are infected and showing symptoms for differential diagnosis.

target gene: highly conserved region of RNA gene sequence of Babesia bovis

Kind of samples: blood samples (blood samples can be shipped throughout the world as one can put 2-3 drops of blood on filter paper as dried blood is sufficient to run the test and send in a letter to any laboratory in the world: It is advisable to put this in extra plastic bag), Tissue and other body fluids. This can be used to screen the bovine serum to be used in cell culture preparation, which can be later on a part of vaccine or monoclonal antibodies production. This needs isolated DNA, which can be isolated with commercial available kit or home made method.

Validation: It has been checked for the cross reactions with related and non related micro organisms like Anaplasma, Neospora caninum, Babesia bigemina, Toxoplasma gondii, DNA of healthy catte, Borrelia burgdorferi, Mycobacterium bovis, Leptospira, Brucella abortus, Theileria etc. The serial dilution has been done to see the detection limit. The specificity of the test is 96-98%.

Detection limit: upto one CPU unit in reaction

Contents: Polymerase, nucleotides, buffer, molecular water, positive control, negative control, loading dye, gene marker (100 bp), PCR master mix and manual.

Storage condition: -20 degree

Genesequencing: optional

only for in vitro use
only for veterinary use
to be used through technical persons.