Technical data sheet (Babesia gibsonii)

Technical details for ready to use PCR kit for Babesia gibsoni (gel agarose based) ; 100 Reactions

Babesia canis and Babesia gibsoni have been infected the dogs and causing the canine babesiosis showing the following symptoms: fever, anorexia, malaise, hemoglobinuria and hemolytica anemia. Babesia canis is reported to cause the disease in Europe, Africa, Asia and America, but the Babesia gibsoni is found in Africa and Asia. Detection of Babesia can be done through microscopic as well as serological methods along with the cell culture methods, but these methods have the problems of lower sensitivity (in case of microscopic diagnosis), cross reactions ( with serological tests) and long duration (with cell culture) needed. Therefore the PCR is merging as gold standard in the field of molecular medicine as it is highly sensitive, more specific and the results can be obtained on the same day or lastest next day. Moreover it needs only small amount of probes.


target gene: highly conserved region of RNA gene sequence of Babesia gibsoni

Kind of samples: blood samples (blood samples can be shipped throughout the world as one can put 2-3 drops of blood on filter paper as dried blood is sufficient to run the test and send in a letter to any laboratory in the world: It is advisable to put this in extra plastic bag), Tissue and other body fluids. This can be used to screen the canine serum as well as cell lines to be used in cell culture preparation, tick (one can isolate the DNA from the tick and run with our PCR to see whether the ticks are infected.)This needs isolated DNA, which can be isolated with commercial available kit or home made method.

Validation: It has been checked for the cross reactions with related and non related micro organisms like Anaplasma, Neospora caninum, Babesia bigemina, Toxoplasma gondii, DNA of healthy dog, Borrelia burgdorferi, Leptospira, Brucella abortus and many more . The serial dilution has been done to see the detection limit. The specificity of the test is 96-98%.

Detection limit: 0.0001% Parasitemia

Contents: Polymerase, nucleotides, buffer, molecular water, positive control, negative control, loading dye, gene marker (100 bp), PCR master mix and manual.

Storage condition: -20 degree

Genesequencing: optional

only for in vitro use
only for veterinary use
to be used through technical persons.