Technical data sheet (Borrelia burgdorferi - Real time)

Technical details for ready to use real time PCR kit for Borrelia burgdorferi (100 Reactions)

Borrelia burgdorferi is a bacterium transmitted through ticks in human beings as well as animals (also birds) like rat, deer, horse, cattle, dog, cat etc. Borrelia burgdorferi is the causative agent of lyme disease in human beings. This bacterium is spread throughout the world. Lyme disease is characterised by some or all of the manifestations: erythematous rash, arthritis, neurological complications as well as / or carditis. Quantitive assays should cover wide range of concentrations since the absolute bacterial load as well as its time course are important diagnostic parameters for indicating the prognosis as well as therapy. Borrelia burgdorferi can be detected through cell culture methods as well as serological methods like FAT, ELISA etc, but these all methods have one or other following drawbacks: low sensitivity, cross reactions or need a long time for detection, therefore PCR is merging as godstandard as this is highly sensitive and finished within a few hours. With real time PCR, one can get the results within 3-4 hours as there is no post PCR stage. In case one can run the known standards with PCR, it can give you the exact number of copies present in the sample. In case the quantititive standard is not available, one can compare 2 samples as one see the bacterial load as curve during real time assay. Real time curves can be helpful during the therapy and predict the success of the treatment.
In case the viral load is going down, it means that the therapy is going to be successful, otherwise one has to think about other alternatives.

target gene: highly conserved region of a Borrelia burgdorferi gene

Samples: blood, serum, plasma, cell culture, tissue and other body fluids. Our test can be used to detect th Borrelia burgdorferi in ticks. It identifies also B.afzelii. It needs the isolated DNA, which can be isolated through commercial kits or home made methods.

Keypoints : It has been checked for cross reactions for related and non related micro organisms like Leptospira, Treponema pallidum, different Babesia, Mycoplasma, negative tick, negative human DNA, negative bird DNA and many more. It has been diluted for serial dilutions.

Detection limit: minimum : 1-3 spirocheat per assay (detection Ct is lower 36 (this should be taken as positive); 36-40 Ct should be taken as weak positive.)

Contents of the kit: Polymerase, buffer, PCR master mix, nucleotides, primers, probes (with 6-carboxyfluoresceine and 6-carboxytetramethylrhodamine), positive control, negative control, molecular water and manual.

Storage: -20 degree

Recommendations: always run the two samples per probe. Species specific internal control can be used.

Only for in vitro use
for veterinary use
only for research use for human probes
to be used through technical person.