Technical data sheet (Chlamydia pneumoniae - Real time)
Technical details for ready
to use real time PCR kit for Chlamydia pneumoniae (100 Reactions)
Chlaymdia pneumoniae is known to cause the respiratory as well as reproductive
diseases in human beings and its role is cardiovascular diseases like
artheroscolesis (????), myocarditits is being discussed in many publications
in the near past. Chlamydiae can be detected through staining methods, cell
culture methods as well as serological methods like FAT, CFT etc, but these
all methods have one or other following drawbacks: low sensitivity, cross
reactions or need a long time for detection, therefore PCR is merging as
godstandard as this is highly sensitive and finished within a few hours. With
real time PCR, one can get the results within 3-4 hours as there is no post
PCR stage. In case one can run the known standards with PCR, it can give you
the exact number of copies present in the sample. In case the quantititive
standard is not available, one can compare 2 samples as one see the chlamydia
load as curve during real time assay. This micro organism has been detected in
animals and there is a need of more research for the role of this bacteria in
animals. Real time PCR can be used during the course of treatment as well as
to see the pharmokinetics of the drug in vitro against the Chlamydia
pneumoniae. During the treatment, if the bacteria load is not going down, it
is the indication of the change of line of treatment.
target gene: highly conserved gene of Chlaymdia pneumoniae
Samples: blood, respiratory swabs, serum, genital swabs, heart and related
blood vessel samples, tissue and other body fluids.It needs the isolated DNA, which can be isolated through
commercial kits or home made methods.
Validation: It has been checked for cross reactions for related and non
related micro organisms like Chlaymdia trachomatis, Chlaymdia psittaci,
Epstein Barr virus, Staphylococcus aerus, Mycoplasma pneumoniae, uninfected
human DNA, Mycoplasma hominis, Legionella and many more. It has been checked
for serial dilutions of Chlamydia pneumoniae.
Detection limit: minimum 50 copies per assay (detectio Ct is lower 36 (this
should be taken as positive); 36-40 Ct should be taken as weak positive.)
Contents of the kit: Polymerase, buffer, PCR master mix, nucleotides, primers,
probes, positive control, negative control, molecular water and manual.
Storage: -20 degree
Only for in vitro use
only for research use
to be used through technical person.