Technical data sheet (Enterovirus)

For 100 reactions

Enteroviruses can cause a wide spectrum of illness ranging from a summer cold to mycocarditis and encephalitis, but most of the infections remains unsymptomatic. When the immune system is intact, it results in the self limiting and it results in the elimination of the virus. Till today one is using cell culture and serological tests along with the dot spot or hybridization assays to detect this virus. These assays have  problem of long time to give the results as well as lower sensitivity  along with the cross reactions problem, therefore the PCR is being used  to get the most accurate results along with the highest specificity and sensitivity. Moreover PCR can be used to detect the different kind of enteroviruses.

Samples: stool, drainage water, drinking water, Throat samples, cell  culture isolation, blood fluids like blood/serum, tissues like in  myocarditis biospies. Our kit needs isolated RNA, therefore one should  use good isolation RNA kit.

Target gene: highly conserved region in the enteroviruses

Validation: tests with related and non related micro organisms. Our kit can identify 60 serotypes of enteroviruses:
Polio 1, 2,3; Cox. A1, A2, A3, A4, A5, A6, A7, A8, A9, A10, A12, A13, A14, A16, A17, A18, A19, A20, A21, A22, Cox. B1, B2, B3, B4, B5, B6, Echo 1,2,3,4,5,6,7,8,9,11,12,13,14,15,17,18,19, 20, 21,24, 25, 26, 27, 29, 30, 31, 32, 33 and Entero 68, 69, 70 and 71 (it does not detect Coxsackie A11, A17 and A24 as well as echovirus 16, 22 and 23)

Sensitivity: 10-50 gemones equivalents

contents of the kit: reverse transcriptase, taq, nucleotides, buffers, primers, negative control, positive control, loading dye, molecular marker, manual etc. The results can be seen in gel agarose.

only for in vitro use
only for research use
To be used by a technical person