Technical data sheet (HBV - Real time)
Technical details for ready
to use real time PCR kit for Human Hepatitis Virus (HBV)
(100 Reactions)
Human Hepatitis B Virus (HBV) is one of the most frequently parenterally
transmitted viruses and needs special diagnostic assays as well as safety
steps for blood and blood products. Quantitive assays should cover wide range
of concentrations since the absolute viral load as well as its time course are
important diagnostic parameters for indicating the prognosis as well as
therapy. HBV can be detected through cell culture methods as well as
serological methods like ELISA etc, but these all methods have one or other
following drawbacks: low sensitivity, cross reactions or need a long time for
detection, therefore PCR is merging as godstandard as this is highly sensitive
and finished within a few hours. With real time PCR, one can get the results
within 3-4 hours as there is no post PCR stage. In case one can run the known
standards with PCR, it can give you the exact number of copies present in the
sample. In case the quantititive standard is not available, one can compare 2
samples as one see the HBV load as curve during real time assay. Real time
curves can be helpful during the antiviral therapy and predict the success of
the treatment.
In case the viral load is going down, it means that the therapy is going to be
successful, otherwise one has to think about other alternatives.
target gene: highly conserved region of HBV gene
Samples: blood, serum, plasma, cell culture, tissue and other body fluids.
This needs isolated DNA, which can be isolated through commercial DNA
isolation kit.
Keypoints : It has been checked for cross reactions for related and non
related micro organisms.
Detection limit: minimum : 100 genome equivalent per assay (detection Ct is
lower 36 (this should be taken as positive); 36-40 Ct should be taken as weak
positive.)
Contents of the kit: Polymerase, buffer, PCR master mix, nucleotides, primers,
probes (with 6-carboxyfluoresceine and 6-carboxytetramethylrhodamine),
positive control, negative control, molecular water and manual.
Storage: -20 degree
Recommendations: always run the two samples per probe.
Only for in vitro use
only for research use
to be used through technical person.