Technical data sheet (Helicobacter pylori)

Ready to use PCR kit for Helicobacter pylori (gel agarose based) for 100 reactions

Helicobacter pylori (HP) is a gram-negative spirally shaped bacterium and is playing a role during the pathogenesis of active chronic gastritis and peptic ulcer disease of humans. It has been also detected in animals like dogs etc. To eradicate this bacterium, one has to identify this in the samples and there are a number of methods available to detect this bacterium like serological methods. The serological methods have the problem of cross reactions as well as low sensitivity. However one can use other methods like Staining and culture methods. The culture method is time consuming as it grows slowly, therefore the method use should be PCR method, where one can hope to detect the bacterium in the smallest samples with highest sensitivity and specificity.

Target gene: highly conserved region of HP

Samples: gastric biopsy, tissue, ulcer, other body fluid samples, bacterial cultures, rectal swabs (in dogs) etc. This assay needs the isolated good quality DNA, which can be isolated with good commercially available kits as well as home made method.

Key point: This assay has been checked for the cross reactions with related and non related micro organisms like Helicobacter mustelae, Campylobacter jejuni, Ureaplasma, Mycoplasma genitalium etc.

Components: primers, polymerase, nucleotides, buffer, molecular water, loading dye, molecular marker (gene ruler), negative control, positive control and manual. In order to use the internal control: one can use the Genekam species specific DNA identification kit for human for human probes or canine for dog probes.

Detection limit: around 10 CFU

Storage: -20 degree

In vitro use only
Only for research use (for human probes)
To be used through technical persons.