Technical data sheet (Human polyoma virus)

100 reactions, gel agarose

The human polyoma virus (DNA virus) are known to infect a high proportion of healthy indivuals usually in the absence of clinical symptoms. In immunocomprised patients, however, these viruses can be reactivated from latency and cause the disease. It may be possible that polyoma viruses can reach brain and result in demyelination due to lytic infection of targeted oligodendrocytes. As the areas of demyelination increase, symptoms of the fatal disorder known as progressive multifocal leukoencephalopathy become more apparent. Similarly, reactivated viral infection can be lead to interstitial nephritis, kidney allograft rejection. Therefore it is essential to detect this virus. In order to detect this virus, one can work with serological methods as well as cell culture methods, but these methods have the problems of cross reactions or need longtime to give the results, therefore one should use PCR method to detect this virus. Moreover PCR method can be finished on the same day.

target gene: highly conserved gene of the virus

samples: human probes, blood, serum/plasma, tissue, nervous tissue, other body fluids like urine etc. Our kit needs isolated DNA, which should be isolated through good quality isolation kit or home made method.

keypoints: It has beeen checked for cross reactions with related and non related viruses and micro organisms like Human Herpes virus, Legionella, HBV etc.

contents of kit: polymerase, nucleotides, buffer, PCR master mix, positive control, negative control, loading dye, molecular marker.

detection limit: 100 genome copies per reaction

storage: -20 degree

only for in vitro use; only for research, only to be used through technical persons .