Technical data sheet (Legionella spp.)

Ready to use PCR kit for Legionella spp. (gel agarose) for 100 reactions

Legionella spp. are gram-negative bacteria responsible to cause respiratory disorders like pneumonia after the inhalation of water droplets. There are around 42 species, which comprise 64 serogroups of legionellae and most of them may cause the disease. About 3-7% community acquired pneumonia is caused through Legionella spp. Particularly elderly and immunocomprised patients are at great risk of death. Clinical presentation of legionellosis involves kidney, liver, gastrointestinal and central nervous system dysfunctions. To eradicate this bacterium, one has to identify this in the samples and there are a number of methods available to detect this bacterium like serological methods. The serological methods have the problem of cross reactions as well as low sensitivity. However one can use other methods like staining and cell culture methods. The culture method is time consuming and needs special medium therefore the method use should be PCR method, where one can hope to detect the bacterium in the smallest samples with highest sensitivity and specificity. One can get the results on the same day.

Target gene: highly conserved region of RNA genes of Legionella: This gene exists as multiple copies per genome.
 
Samples: blood, serum, plasma, respiratory swabs, tissue, other body fluid samples, cell cultures, water probes, drainage probes etc.. This assay needs the isolated good quality DNA, which can be isolated with good commercially available kits as well as home made method.

Key point: This assay has been checked for the cross reactions with related and non related micro organisms like Staphylococcus, Candida albicans, Haemophilus influenzae, Bordetella, Chlamydia pneumoniae, Mycoplasma pneumoniae etc.

This assay can detect clinical relevant Legionella spp. like L. pneumophila, L. micdalei, L. longbeachae, L. bozemanii, L. feacalis and L. dumoffii etc.

Components: primers, polymerase, nucleotides, buffer, molecular water, loading dye, molecular marker (gene ruler), negative control, positive control and manual. In order to use the internal control: one can use the Genekam species specific DNA identification kit for human for human probes or other species.

Detection limit: around 2 CFU (10 fg) per reaction

Storage: -20 degree

In vitro use only
Only for research use (for human probes)
To be used through technical persons.