Technical data sheet (Mycobacterium avium sp. paratuberculosis)

Techincal details of Ready to use PCR kit (duplex: double check) Mycobacterium avium sp. paratuberculosis (gel agarose based): 100 reactions

Mycobacterium avium sp. paratuberculosis (MAP) is known to cause the chronic disease called Johne´s disease in domestic as well as wild ruminants. The symptoms of the disease are chronic diarrhoae along with decreased milk production, aneamia, infertility and even death in final stage of disease. The infected animals are shedding heavy number of bacteria in the feaces and contaminating the enviroment, therefore it is essential to remove the diseased or carrier animals. It is also shed in milk. MAP is known to play a role in human beings suffering from immunosuppressive diseases like HIV as the presence of MAP can complicate the case of such patients. Mycobacteria are Gram positive and acid-fast bacteria. Because of subclinical infections, this pathogen has been undiagnosed for serval years. There is a discussion going about the role of MAP in Crohn´s disease in human beings, hence making this bacterium as an important food safety corcern. There are also reports that MAP can survive some form of pasteurization, therefore there is a need of affective diagnostic as well as control measurements of this bacterium. Therefore it is essestial to detect this bacterium as early as possible. In order to detect this, there are different methods like staining, culture growth as well as serolgical methods available, but these methods have problems like low sensitivity, needs very long time as well as cross reactions etc, therefore PCR method is merging as the gold standard as it is highly sensitive, highly specific and results can be obtained in 24 hours. In case of MAP, many laboratories are targetting the genesequence IS900 of MAP to detect this as this IS900 has been found in other mycobacteria species, hence it leads to cross reactions and lower specificity. Even there are reports of identication of enviromental mycobacteria with IS900 PCR, therefore Genekam Biotechnology AG decided to bring the PCR kit on the market to target 2 different genes in order to give the most accurate detections for MAP as PCR with only IS900 may give false results or results of IS900 PCR should be taken with caution. Even this problem can not be solved with restriction endonucleasis analysis. Hence the ready to use PCR (double check) for MAP is the answer.

target genes: 2 different sets including IS of gene of MAP.

Samples: feaces, blood, milk, tissue, digestive tract, lymphnodes and other body parts. This kit needs isolated good DNA, which can done through commercial available kit or home made method.

Validation: It has been checked for cross reactions with related or non related micro organisms: different Mycobacteria, Borrelia burgdorferi, Leptospira, Coxiella burnetii, Bovine Herpes virus and many more. It has been checked for serial dilutions. It has been checked to detect the MAP strains from different cattles.

Detectionlimit: 1CFU per PCR reaction for each target

Contents: Polymerase, nucleotides, buffer, molecular water, positive control, negative control, loading dye, gene marker (100 bp), PCR master mix and manual.

Storage condition: -20 degree

Genesequencing: optional

only for in vitro use
only for veterinary use
to be used through technical persons.