Technical data sheet
Real time PCR kit for Mycobacterium tuberculosis (Simplex and duplex version)
Tuberculosis (TB) is one of major public health problem throughout the world.
In order to overcome this disease, one has to develop rapid, sensitive and
specific identifcation methods. Traditional laboratory methods such direct
microscopy and culture methods have drawbacks in view of identification of
Mycobacterium tuberculosis as they are not so sensitive or they need skilled
manpower (who give again different results of the same probe) or need a long
time. The detection of Mycobacterium tuberculosis DNA sequence might be
representing a more sensitive and fast daignostic target. One needs efficient
method to detect the Mycobacterium tuberculosis in sputum and moreover to
monitor the drug therapy, there is need of quantitative PCR assay. At present,
the laboratories are using the PCR assay, which detects only one target gene
and there are a certain strains of Mycobacterium tuberculosis, which does not
contain this strain, therefore they can escape this test, therefore Genekam
has brough a ready to use PCR kit, which can detect one most common gene
target (which is widely accepted as standard throughout the world in all
laboratories carrying the identification of Mycobacterium tuberculosis) and an
extra gene, which is available in certain strains without the most common gene
target.
However Genekam offers the assay in two forms: 1. ready to use kit for one
target gene
2. ready to use kit for detectiong 2 different target genes
target gene: one IS gene (multicopy gene) in simplex version and two highly
conversed gene targets (one is IS: it is multicopy gene and an other gene) in
duplex version
Types of the samples: Sputum, strains from the culture, Respiratory fluid,
lung, tissue, blood etc. DNA can be isolated through commecial available kits
or home made good methods. This kit need good quality of DNA.
Contents of Kit: primern, fluorogenic primers (filter FAM to used in one step
PCR and filters FAM and VIC are to be used in 2 step PCR), nucleotides,
buffers, molecular water, negative control, positive control and manual. Dyes
used are 6-carboxy-fluorscein, YYE and 6-carboxytetramethylrhodamine.
Validations: The assay has been validated for cross reactions with related
like Mycobacterium avium sp paratuberculosis, Mycobacteriu avium sp avium etc
and non related micro organisms like Salmonella, Legionella, Staphyloccus etc.
along with the different viruses of human and animal origin. The assy is 98%
sensitive in most common used IS gene against the microscopic method.
detectionlimit: 10-50 genome equivalent per reaction
Storage: -20 degree
only for in vitro use; only for research use; to be used through technical
person