Technical details for ready to use PCR kit for Mycoplasma agalactiae (gel agarose based; 100 reactions)

Mycoplasma agalactiae is a pathogenic mycoplasma causing contagious agalactia in small ruminants like sheep. This shows acute as well as subacute clinical symptoms like masitis, keratitis, arthritis etc., therefore there is a need of laboratory diagnosis. This laboratory diagnosis can be done through culture methods (which takes a longtime as Mycoplasma goes slowly and needs special media), serological methods like ELISA as well as FAT, which are prone to cross reactions, therefore the PCR is merging as a goldstandard for detection of micro organisms in medicine as this is highly sensitive and specific also. Moreover PCR needs a little material to conduct the test.

Target gene: highly conserved region of gene of Mycoplasma agalactiae

Kinds of samples: milk, colonies grown on cultures, blood (serum/plasma) samples, tissue samples and other body fluid samples. This kit needs isolated DNA, which can be isolated using commercial available kits or home made methods.

Components of the kit: polymerase, forward and backward primers, nucleotides, buffers, PCR master mix, loading dye, gene ruler (100 bp), positive control, negative control as well as manual.

Validations: It has been tested for cross reactions with related and non related micro organisms like Mycoplasma bovis, Mycoplasma mycoides, different Staphylocci, Staphylococcus aereus, different Streptococci, E.coli and many more . It is tested for serial dilutions.

Sensitivity: 2.5-25 fg of Mcoplasma agalactiae

Only for in vitro use
Only for veterinary use
to be used through technical person