Technical details for ready to use PCR kit for Mycoplasma hominis (gel agarose based; 100 reactions)
Mycoplasma hominis is a pathogenic mycoplasma,
which has been isolated from the urogenital tract of 40% asymptomatic males as
well as females in human beings. It is a proven cause of pelvic inflammatory
disease, postpartum septicemia and endocarditis, clinical amnionitis and
pyelonephritis. It is the major cause of pnemonia and central nervous system
infections in new borne infants. It is being recognised a common cause of
septicemia, arthritis, surgical wound infections as well as peritonitis in
immunocompromised patients, therefore there is a need of laboratory diagnosis.
This laboratory diagnosis can be done through culture methods (which takes a
longtime as Mycoplasma goes slowly and needs special media), serological
methods like ELISA as well as FAT, which are prone to cross reactions,
therefore the PCR is merging as a goldstandard for detection of micro
organisms in medicine as this is highly sensitive and specific also. Moreover
PCR needs a little material to conduct the test.
Target gene: highly conserved region of gene of Mycoplasma hominis.
Kinds of samples: genital swabs, cervix swabs, respiratory system swabs,
cardiac probes, wound swabs, peritoneal fluid swabs, colonies grown on
cultures, systematic swabs, blood samples, tissue samples and other body fluid
samples. This kit needs isolated DNA, which can be isolated using commercial
available kits or home made methods.
Components of the kit: polymerase, forward and backward primers, nucleotides,
buffers, PCR master mix, loading dye, gene ruler (100 bp), positive control,
negative control as well as manual.
Validations: It has been tested for cross reactions with related and non
related micror organisms like Mycoplasma pneumoniae, Mycoplasma genitalium,
Coxiella burnetii, Staphylococcus aureus, Bordetella pertusis, Bordetella
parapertusis, Chlamydia pneumoniae, healthy human DNA, Cytomegalovirus and
many more. It is tested for serial dilutions.
Sensitivity: 10-15 fg of Mycoplasma hominis
Only for in vitro use
Only for research use
to be used through technical person