Technical details for ready to use PCR kit for Mycoplasma hyorhinis (gel agarose based; 100 reactions)

Mycoplasma hyorhinis is a pathogenic mycoplasma, which causes respiratory disorders (called enzootic pig pneumonia: a chronic respiratory disease) in pigs world wide along with M. hyopneumoniae. This is characherized by high morbidity rates as well as low mortality leading to economic losses through retarded growth, poor food conversion and increased susceptibility of pigs to other infections, therefore there is a need of laboratory diagnosis. This laboratory diagnosis can be done through culture methods (which takes a longtime as Mycoplasma goes slowly and needs special media), serological methods like ELISA as well as FAT, which are prone to cross reactions, therefore the PCR is merging as a goldstandard for detection of micro organisms in medicine as this is highly sensitive and specific also. Moreover PCR needs a little material to conduct the test. Moreover PCR can be used to differentiate between the different strains of M. hoypneumoniae, M. hyorhinis and M. flocculare.

Target gene: highly conserved region of gene of Mycoplasma hyorhinis.

Kinds of samples: respiratory system swabs, nasal swabs, colonies grown on cultures, blood (serum/plasma) samples, tissue samples and other body fluid samples. This kit needs isolated DNA, which can be isolated using commercial available kits or home made methods.

Components of the kit: polymerase, forward and backward primers, nucleotides, buffers, PCR master mix, loading dye, gene ruler (100 bp), positive control, negative control as well as manual.

Validations: It has been tested for cross reactions with related and non related micror organisms like Mycoplasma hyopneuomiae, Chlamydia psittaci, healthy pig DNA, Acholeplasma laidlawii and many more. It is tested for serial dilutions.

Sensitivity: 1-50 pg of extracted Mycoplasma hyorhinis DNA

Only for in vitro use
Only for veterinary use
to be used through technical person