Technical details for ready to use PCR kit for Mycoplasma conjunctivae (gel agarose based; 100 reactions)

Mycoplasma conjunctivae is a pathogenic mycoplasma, which causes infectious keratoconjunctivitis (also known as pink eye) in domestic ruminants and it is characterized by inflammation of the conjunctiva and cornea. It causes this disease in sheep and goat leading ecomonical losses therefore there is a need of laboratory diagnosis. This laboratory diagnosis can be done through culture methods (which takes a longtime as Mycoplasma goes slowly and needs special media), serological methods like ELISA as well as FAT, which are prone to cross reactions, therefore the PCR is merging as a goldstandard for detection of micro organisms in medicine as this is highly sensitive and specific also. Moreover PCR needs a little material to conduct the test.

Target gene: highly conserved region of gene of Mycoplasma conjunctivae.

Kinds of samples: eye swabs, colonies grown on cultures, systematic swabs, blood samples, tissue samples and other body fluid samples. This kit needs isolated DNA, which can be isolated using commercial available kits or home made methods.

Components of the kit: polymerase, forward and backward primers, nucleotides, buffers, PCR master mix, loading dye, gene ruler (100 bp), positive control, negative control as well as manual.

Validations: It has been tested for cross reactions with related and non related micror organisms like Mycoplasma agalactiae, Chlamydia psittaci, healthy sheep DNA, healthy goat DNA and many more. It is tested for serial dilutions.

Sensitivity: 20 CFU

Only for in vitro use
Only for veterinary use
to be used through technical person