Technical details for ready to use PCR kit for Mycoplasma gallisepticum (gel agarose based; 100 reactions)

Mycoplasma gallisepticum is a pathogenic mycoplasma, which causes respiratory diseases in chickens as well as infectious sinusitis in turkeys. The transmission of M. gallisepticum can be vertical (direct between the infected and suspectible birds) or horizontal. Moreover indirect transmission through human as well as formites play an important role because of persistence of Mycoplasmas in enviroment therefore there is a need of laboratory diagnosis. This laboratory diagnosis can be done through culture methods (which takes a longtime as Mycoplasma goes slowly and needs special media), serological methods like ELISA as well as FAT, which are prone to cross reactions, therefore the PCR is merging as a goldstandard for detection of micro organisms in medicine as this is highly sensitive and specific also. Moreover PCR needs a little material to conduct the test. Usually there is a coexistence of Mycoplasma gallisepticum and Mycoplasma synoviae. Serological tests may not be able to do this differentiate. Mycoplasmas causes economical losses in poultry industry. Mycoplasma has been reported in many other species of birds like parrots etc.

Target gene: highly conserved region of RNA gene of Mycoplasma gallisepticum.

Kinds of samples: Throat swabs, respiratory system swabs, colonies grown on cultures, reproductive tract swabs, blood samples, tissue samples and other body fluid samples. This kit needs isolated DNA, which can be isolated using commercial available kits or home made methods.

Components of the kit: polymerase, forward and backward primers, nucleotides, buffers, PCR master mix, loading dye, gene ruler (100 bp), positive control, negative control as well as manual.

Validations: It has been tested for cross reactions with related and non related micror organisms like Mycoplasma synoviae, Mycoplasma mealegridid, Mycoplasma iowae, Chlamydia psittaci and many more. It is tested for serial dilutions.

Sensitivity: 0.02 CFU

Only for in vitro use
Only for veterinary use
to be used through technical person