Technical data sheet (NDV)

Technical details of ready to use PCR kit for New castle disease virus (NDV)

100 reactions gel agarose

New castle disease virus is RNA virus affecting the poultry as well as other birds. Infected birds present respiratory syndromes and other lesions like cough as well as poor growth leading to economic losses. Mixes infection with other micro organisms like pathogenic Mycoplasma , Infectious laryngotracheatis virus, infectious bronchitis viruses may occur because of use of multiple live vaccines and kind of housing. Diagnostic methods like serology and isolation are used for detecting these viruses, but they have problems of cross reactions (in serology) and time consuming in case of cell culture isolations, therefore molecular methods like PCR are methods of choice as they are fastest and highly sensitive along with highly specific.

target gene: highly conversed gene

samples: respiratory swabs, blood, serum, tissue and other body fluids along with cell cultures as well as allantoic fluids from embryonated eggs.

Contents of PCR kit: nucleotides, reverse transcriptase, buffer, molecular water, polymerase, loading dye, molecular marker, positive control, negative control and manual

Detection limit: 10-100 pg in one reaction

Storage at -20 degree

Only for in vitro use; only for veterinary use; to be used through technical persons.